TitleVariation in expression of Biomphalaria glabrata SOD1: A potential controlling factor in susceptibility/resistance to Schistosoma mansoni
Publication TypeJournal Article
Year of Publication2007
AuthorsBender, RC, Goodall, CP, Blouin, M, Bayne, CJ
JournalDevelopmental and Comparative Immunology
Type of ArticleJournal Article

The snail Biomphalaria glabrata kills the blood fluke Schistosoma mansoni by a mechanism involving production of hydrogen peroxide, the enzymatic product of cytosolic Cu/Zn superoxide dismutase (SODI). This enzyme exhibits higher activity in blood cells (hemocytes) from a predominantly resistant strain of B. glabrata than in hemocytes from a susceptible strain. Additionally, B. glabrata SODI polymorphisms have been associated with susceptibility/resistance to the parasite. To address the hypothesis that SODI transcription levels differ in accordance with variation at the SODI locus, quantitative PCR was performed using hemocyte-derived cDNA prepared from SODI-genotyped snails. Here we report that individuals possessing the allele previously associated with resistance to S. mansoni express significantly higher levels of hemocyte SODI transcripts than individuals lacking this allele. A causal relationship between SODI expression and susceptibility/resistance to S. mansoni is supported by the correlation of transcript quantity with data (from a previous study) on the probability of infection. (C) 2007 Published by Elsevier Ltd.

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