Title | Variation in expression of Biomphalaria glabrata SOD1: A potential controlling factor in susceptibility/resistance to Schistosoma mansoni |
Publication Type | Journal Article |
Year of Publication | 2007 |
Authors | Bender, RC, Goodall, CP, Blouin, M, Bayne, CJ |
Journal | Developmental and Comparative Immunology |
Volume | 31 |
Pagination | 874-878 |
Type of Article | Journal Article |
ISSN | 0145-305X |
Abstract | The snail Biomphalaria glabrata kills the blood fluke Schistosoma mansoni by a mechanism involving production of hydrogen peroxide, the enzymatic product of cytosolic Cu/Zn superoxide dismutase (SODI). This enzyme exhibits higher activity in blood cells (hemocytes) from a predominantly resistant strain of B. glabrata than in hemocytes from a susceptible strain. Additionally, B. glabrata SODI polymorphisms have been associated with susceptibility/resistance to the parasite. To address the hypothesis that SODI transcription levels differ in accordance with variation at the SODI locus, quantitative PCR was performed using hemocyte-derived cDNA prepared from SODI-genotyped snails. Here we report that individuals possessing the allele previously associated with resistance to S. mansoni express significantly higher levels of hemocyte SODI transcripts than individuals lacking this allele. A causal relationship between SODI expression and susceptibility/resistance to S. mansoni is supported by the correlation of transcript quantity with data (from a previous study) on the probability of infection. (C) 2007 Published by Elsevier Ltd. |
URL | <Go to ISI>://WOS:000247553800002 |
DOI | 10.1016/j.dci.2006.12.005 |