TitleSurvival of Swiss-Webster mouse cerebellar granule neurons is promoted by a combination of potassium channel blockers
Publication TypeJournal Article
Year of Publication2007
AuthorsCollins, A, Larson, MK, Pfaff, JE, Ishmael, JE
JournalToxicology Letters
Volume171
Pagination60-68
Type of ArticleJournal Article
ISSN0378-4274
Abstract

Cultured cerebellar granule neurons (CGN) are commonly used to assess neurotoxicity, but are routinely maintained in supraphysiological (25 mM) extracellular K+ concentrations [K+](o). We investigated the effect of potassium channel blockade on survival of CGN derived from Swiss-Webster mice in supraphysiological (25 mM) and physiological (5.6 mM) [K+](o). CGN were cultured for 5 days in 25 mM K+, then in 5.6 mM K+ or 25 mM K+ (control). Viability, assayed 24 h later by 3-(4,5-dimethylthiazolyl)2,5-diphenyltetrazolium bromide (MTT) reduction and by lactate dehydrogenase (LDH) release, was similar to 50% in 5.6 mM K+ versus 25 mM K+ (p .001), but nifedipine did not attenuate neuroprotection by K+ channel blockers. Together, these results suggest that the survival of CGN depends on the K+ permeability of the membrane rather than the activity of a particular type of K+ channel, and that the mechanism of neuroprotection by K+ channel blockers, is different from that of elevated [K+](o). (c) 2007 Elsevier Ireland Ltd. All rights reserved.

URL<Go to ISI>://WOS:000248467000007
DOI10.1016/j.toxlet.2007.04.004