TitleSurvival of Swiss-Webster mouse cerebellar granule neurons is promoted by a combination of potassium channel blockers
Publication TypeJournal Article
Year of Publication2007
AuthorsCollins, A, Larson, MK, Pfaff, JE, Ishmael, JE
JournalToxicology Letters
Type of ArticleJournal Article

Cultured cerebellar granule neurons (CGN) are commonly used to assess neurotoxicity, but are routinely maintained in supraphysiological (25 mM) extracellular K+ concentrations [K+](o). We investigated the effect of potassium channel blockade on survival of CGN derived from Swiss-Webster mice in supraphysiological (25 mM) and physiological (5.6 mM) [K+](o). CGN were cultured for 5 days in 25 mM K+, then in 5.6 mM K+ or 25 mM K+ (control). Viability, assayed 24 h later by 3-(4,5-dimethylthiazolyl)2,5-diphenyltetrazolium bromide (MTT) reduction and by lactate dehydrogenase (LDH) release, was similar to 50% in 5.6 mM K+ versus 25 mM K+ (p .001), but nifedipine did not attenuate neuroprotection by K+ channel blockers. Together, these results suggest that the survival of CGN depends on the K+ permeability of the membrane rather than the activity of a particular type of K+ channel, and that the mechanism of neuroprotection by K+ channel blockers, is different from that of elevated [K+](o). (c) 2007 Elsevier Ireland Ltd. All rights reserved.

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