TitleThe relative roles of three DNA repair pathways in preventing Caenorhabditis elegam mutation accumulation
Publication TypeJournal Article
Year of Publication2006
AuthorsDenver, DR, Feinberg, S, Steding, C, Durbin, M, Lynch, M
JournalGenetics
Volume174
Pagination57-65
Type of ArticleJournal Article
ISSN0016-6731
Abstract

Mutation is a central biological process whose rates and spectra are influenced by a variety of complex and interacting forces. Although DNA repair pathways are generally known to play key roles in maintaining genetic stability, much remains to be understood about the relative roles of different pathways in preventing the accumulation of mutations and the extent of heterogeneity in pathway-specific repair efficiencies across different genomic regions. In this study we examine mutation processes in base excision repair-deficient (nth-1) and nucleotide excision repair-deficient (xpa-1) Caenorhabditis elegans mutation-accumulation (MA) lines across 24 regions of the genome and compare our observations to previous data from mismatch repair-deficient (msh-2 and msh-6) and wild-type (N2) MA lines. Drastic variation in both average and locus-specific mutation rates, ranging two orders of magnitude for the latter, was detected among the four sets of repair-deficient MA lines. Our work provides critical insights into the relative roles of three DNA repair pathways in preventing C. elegans mutation accumulation and provides evidence for the presence of pathway-specific DNA repair territories in the C. elegans genome.

URL<Go to ISI>://WOS:000241134400006
DOI10.1534/genetics.106.059840