TitleGenetic variation in two populations of the rough-skinned newt (Taricha granulosa) assessed using novel tetranucleotide microsatellite loci
Publication TypeJournal Article
Year of Publication2001
AuthorsJones, AG, Blouin, M, Arnold, SJ
JournalMolecular Ecology Notes
Volume1
Pagination293-296
Keywordshoming microsatellites newts parentage population structure site fidelity
Abstract

Seven novel tetranucleotide microsatellite loci were identified from a partial genomic DNA library, enriched for GATA-motif microsatellites, from the rough-skinned newt (Taricha granulosa). All loci were polymorphic, and one displayed a high frequency null allele. A related species, T. rivularis, displays strong site fidelity and detectable population structure over small spatial scales, so we assessed genetic variation in two samples of T. granulosa separated by 16 km. Distributions of allele frequencies differ significantly between our two sites, but small values of F-ST and Rho(ST) suggest that the populations are linked by a large amount of gene flow.