TitleDisruption of sperm release from insect testes by cytochalasin and beta-actin mRNA mediated interference
Publication TypeJournal Article
Year of Publication2003
AuthorsGvakharia, BO, Bebas, P, Cymborowski, B, Giebultowicz, J
JournalCellular and Molecular Life Sciences
Volume60
Pagination1744-1751
Type of ArticleJournal Article
ISSN1420-682X
Abstract

Release of sperm bundles from moth testes is controlled by the local circadian oscillator. The mechanism which restricts migration of sperm bundles to a few hours each day is not understood. We demonstrate that a daily cycle of sperm release is initiated by the migration of folded apyrene sperm bundles through a cellular barrier at the testis base. These bundles have conspicuous concentrations of actin filaments at their proximal end. Inhibition of actin polymerization by cytochalasin at a specific time of day inhibited sperm release from the testis. Likewise, application of double-stranded actin RNA specifically inhibited sperm release. This RNA-mediated interference (RNAi) lowered the pool of actin mRNA in tissues involved in sperm release. The decline in mRNA levels resulted in the selective depletion of F-actin from the tip of apyrene sperm bundles, suggesting that this actin may be involved in the initiation of sperm release. Combined results of RNAi experiments at physiological, cellular and molecular levels identified unique cells that are critically involved in the mechanism of sperm release.

URL<Go to ISI>://WOS:000185201300019
DOI10.1007/s00018-003-3139-z