TitleCyclophilin and the regulation of symbiosis in Aiptasia pallida
Publication TypeJournal Article
Year of Publication2008
AuthorsPerez, S, Weis, VM
JournalBiological Bulletin
Volume215
Pagination63-72
Type of ArticleJournal Article
ISSN0006-3185
Abstract

The sea anemone Aiptasia pallida, symbiotic with intracellular dinoflagellates, expresses a peptydylprolyl cis-trans isomerase (PPIase) belonging to the conserved family of cytosolic cyclophilins (ApCypA). Protein extracts from A. pallida exhibited PPIase activity. Given the high degree of conservation of ApCypA and its known function in the cellular stress response, we hypothesized that it plays a similar role in the cnidarian-dinoflagellate symbiosis. To ex lore its role, we inhibited the activity of cyclophilin with cyclosporin A (CsA). CsA effectively inhibited the PPIase activity of protein extracts from symbiotic A. pallida. CsA also induced the dose-dependent release of symbiotic algae from host tissues (bleaching). Laser scanning confocal microscopy using superoxide and nitric oxide-sensitive fluorescent dyes on live specimens of A. pallida revealed that CsA strongly induced the production of these known mediators of bleaching. We tested whether the CsA-sensitive isomerase activity is important for maintaining the activity of the antioxidant enzyme superoxide dismutase (SOD). SOD activity of protein extracts was not affected by pre-incubation with CsA in vitro.

URL<Go to ISI>://WOS:000258845100007