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|Title||2b-RAD: a simple and flexible method for genome-wide genotyping|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Wang, S, Meyer, E, McKay, JK, Matz, MV|
|Type of Article||Journal Article|
We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput genotyping as required for linkage mapping and profiling genetic variation in natural populations.
|URL||<Go to ISI>://WOS:000307015700021|