OREGON STATE UNIVERSITY
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| Title | 2b-RAD: a simple and flexible method for genome-wide genotyping |
| Publication Type | Journal Article |
| Year of Publication | 2012 |
| Authors | Wang, S, Meyer, E, McKay, JK, Matz, MV |
| Journal | Nature Methods |
| Volume | 9 |
| Pagination | 808-+ |
| Type of Article | Journal Article |
| ISSN | 1548-7091 |
| Abstract | We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput genotyping as required for linkage mapping and profiling genetic variation in natural populations. |
| URL | <Go to ISI>://WOS:000307015700021 |
| DOI | 10.1038/nmeth.2023 |