Title2b-RAD: a simple and flexible method for genome-wide genotyping
Publication TypeJournal Article
Year of Publication2012
AuthorsWang, S, Meyer, E, McKay, JK, Matz, MV
JournalNature Methods
Volume9
Pagination808-+
Type of ArticleJournal Article
ISSN1548-7091
Abstract

We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput genotyping as required for linkage mapping and profiling genetic variation in natural populations.

URL<Go to ISI>://WOS:000307015700021
DOI10.1038/nmeth.2023