Homebrew-RNALater

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Objective

A homemade RNA stabilization buffer similar to RNALater. Recipe [1] from the Palumbi Lab at Stanford.


Step by step instructions

Component Amount
Ammonium sulfate 117 g
0.75 M Sodium citrate 5.556 ml
0.5 M EDTA 6.667 ml

  1. Add ~100 ml NFW to clean reagent jar
  2. Add ammonium sulfate and heat to dissolve
  3. Add liquid components
  4. Bring total volume to within 10 ml of the final volume (250 ml)
  5. pH with sulfuric acid to pH 5.2
  6. add NFW to final volume of 250 ml
  7. store at room temperature

History

Created 09:22 Apr 01, 2016 By: EliMeyer

Last updated 04:59 Jun 26, 2019 By: Admin